8.0 Lymh Nodes Biopsy


Title of Manual: Specimen Collection

Version No: 1.0

Title:Lymph Node Biopsy – for Lymphoma
Infection or Metastatic Malignancy

Effective Date: 11/01/09
Revised Date: 02/27/14

Document #: GPA.SPC.8.0


I. PURPOSE

To provide directions for the proper collection of Lymph Node specimen(s) for testing and pathology review.

II. MATERIALS

Reagents

Supplies

Equipment

• Sterile Container (no Formalin)
• Sterile container with 10% Neutral Buffered Formalin


III. PROCEDURE

Lymph node samples are submitted fresh so that portions may be obtained for microbiology studies, frozen section, immunoperoxidase, and for molecular biologic studies (DNA) gene rearrangement assay. Seal the container and keep refrigerated until pick up. DO NOT FREEZE.

Lymph nodes submitted for routine Histology only (requiring no special studies) must be submitted in
ten percent neutral buffered formalin solution. Formalin containers must have a formalin hazard label on
the outside of the container. The amount of formalin should be fifteen to twenty times the volume of
the specimen.

Please see requisition form instructions for complete information. Complete test requisition including last and first name of patient, patient’s date of birth and social security number, body site and source of specimen collected. Label specimen container (using labels provided on the requisition) with the patient’s first and last name, and body site/source. Include pertinent clinical information, i.e., previous malignancy, radiation therapy, drugs, etc. Place container in a specimen bag with a biohazard label. Place the requisition in the side pocket of the specimen bag.

IV. QUALITY CONTROL

• Minimum Volume:
     • 0.1 X 0.1 X 0.1 cm
• Storage Requirements:
     • If specimen is to be submitted fresh, keep refrigerated until pick up. DO NOT FREEZE. Place
       the specimen on saline moistened gauze in a sterile specimen cup for transport. Submit specimen on
       wet ice.
     • For routine histology, store above freezing in 10% Neutral Buffered Formalin.
• Stability Requirements:
     • Fresh tissue, submit to the laboratory within 2-3 hours fresh (dampened in sterile saline) or 24 hours
       in transport media.

V. CALCULATIONS/CALIBRATION

VI. INTERPRETATIONS

VII. METHOD PERFORMANCE SPECIFICATIONS

VIII. REFERENCES

IX. RELATED DOCUMENTS

X. DOCUMENT HISTORY

MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

X - MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

2/27/14 New Document Control Format



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date


(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

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(Signature)



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