7.0 Direct Immunofluorescence Studies-IgG, IgA, C3


Title of Manual: Specimen Collection

Version No: 1.0

Title: 7.0 Direct Immunofluorescence
Studies-IgG, IgA, C3

Effective Date: 11/01/09
Revised Date: 06/23/14

Document #: GPA.SPC.7.0


I. PURPOSE

To provide directions for the proper collection of tissue specimen(s) for pathology review.

II. MATERIALS

Reagents

Supplies

Equipment

• Sterile container
with Michel’s Fixative


III. PROCEDURE

The specimen must be submitted in Michel’s Fixative. Michel’s containers must have a hazard label on the outside of the container.

Please see requisition form instructions for complete information. Complete test requisition including last and first name of patient, patient’s date of birth and social security number, body site and source of specimen collected. Label specimen container (using the labels provided on the requisition) with patient’s first and last name, and body site/source. Check the box on the requisition for Immunofluorescence. Include pertinent clinical information, i.e., previous malignancy, radiation therapy, drugs, etc. Place container in a specimen bag with a biohazard label. Place the requisition in the side pocket of the specimen bag.

IV. QUALITY CONTROL

• Minimum Volume:
     • 0.1 X 0.1 X 0.1 cm
• Storage Requirements:
     • Refrigerate, if possible. Best if kept cool (wet ice in cooler). Michel’s Fixative for transport.
• Stability Requirements:
     • 3 days in Michel’s Fixative

V. CALCULATIONS/CALIBRATION

VI. INTERPRETATIONS

VII. METHOD PERFORMANCE SPECIFICATIONS

VIII. REFERENCES

IX. RELATED DOCUMENTS

X. DOCUMENT HISTORY

MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

X - MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

06/23/14 New Document Control Format



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date


(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)



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