58.0 Culture Collection


Title of Manual: Specimen Collection

Version No: 1.0

Title: Culture Collection

Effective Date: 02/27/14
Revised Date:

Document #: GPA.SPC.58.0


I. PURPOSE

To provide directions for the proper collection of culture specimens to ensure optimal collection techniques and viable results.

II. MATERIALS

Reagents

Supplies

Equipment

 

• Sterile Leak Proof Container
• Anaerobic Transport Gel Vial
• Culture Transport Swab

 


III. PROCEDURE

A. Aerobic Culture: superficial wound, abscess, aspirates, animal bites, skin biopsy, tissue samples
      1. Tissue or aspirated fluid is superior to a swab specimen.
      2. Cleanse an area of 1 -2 cm with sterile saline before sampling the area.
      3. Using sterile techniques aspirate if possible or pass a swab deep into the lesion and firmly sample
          the advancing edge of the lesion.
      4. Place the swab in the transport device and deliver to the laboratory immediately.
      5. Tissue samples should be removed aseptically and placed in sterile leak proof container with a small
          amount of sterile saline to keep the tissue sample from drying out or in a culture transport medium.
      6. Stable at room temperature for 48 hours, refrigerated for 24 hours.
      7. Frozen specimens are not acceptable for testing.
B. Anaerobic Culture: abscess or deep wound, tissue, normally sterile body fluids other than blood,
     transtracheal aspirations, suprapubic urine aspirates obtained from bladder, exudates, aspirated pus
     from deep wound or abscesses and tympanocentesis fluid.
      1. Tissue or aspirated fluid is superior to a swab specimen.
      2. Cleanse an area of 1 -2 cm with sterile saline before sampling the area.
      3. Using sterile techniques aspirate if possible or pass a swab deep into the lesion and firmly sample
          the advancing edge of the lesion.
      4. Place the swab in the transport device and deliver to the laboratory immediately.
      5. Tissue samples should be removed aseptically and placed in an anaerobic transport system or an           anaerobic transport gel vial.
      6. Stable at room temperature for 48 hours.
      7. Refrigerated and Frozen specimens are not acceptable for testing.
C. Fungus Culture: mouth, nose, nasopharynx, ear, eye, wound, vagina, cervix, urethra, body fluids, tissue,      skin, hair, nails, sputum, bone marrow, urine, stool, biopsy.
      1. Skin, Hair, Nails:
            a. Cleanse the skin with alcohol before scraping.
            b. Remove hairs with forceps, scrape skin or scalp scales, clip nails, and include keratin scrapings.
            c. Stable at room temperature and refrigerated for 2 weeks
            d. Frozen specimens are not acceptable for testing.
      2. Specimens other than skin, hair, nails:
            a. Swab the affected site using sterile techniques.
            b. Place the swab in the transport device and deliver to the laboratory immediately.
            c. Submit sterile body fluids in a sterile, leakproof container or vial.
            d. Stable at room temperature and refrigerated for 72 hours.
            e. Frozen specimens are not acceptable for testing.
D. Urine Culture:
      1. Instructions for Female Patient:
            a. Remove all necessary garments.
            b. Wash hands thoroughly with soap and warm water, rinse and dry hands.
            c. Separate the skin folds around the urinary opening and clean this area with the provided cleaning
                towelette. Clean the opening and the surrounding area in a front to back downward motion.
            d. Discard the towelette in the trash.
            e. Repeat the cleansing process 2 more times using clean towelettes each time. Remember to hold the
                skin folds apart while cleansing the area.
            f. Holding the skin folds apart with one hand, begin to urinate into the toilet, and then stop urinating
               and place the specimen cup into position and begin urinating again. It is extremely important not to
               touch the inside or the lip of the container with your hands or any part of the body.
            g. After specimen is collected, secure the lid on tightly and give the specimen to the lab personnel.
                     i. The patient should be instructed not to touch the interior of the top or container.
                    ii. Label the cup with the patient information.
                   iii. Urinate into the urine specimen container.
                   iv. Collect the sample until the cup is about ¾‘s full.
                    v. Transfer sample to gray top urine culture tube labeled with patient information.
      2. Instructions for the Male Patient:
            a. Remove all necessary garments.
            b. Wash hands thoroughly with soap and water, rinse and dry hands.
            c. Pulling the foreskin back with one hand, clean the head of the penis with the provided cleansing
                towelette. Clean the opening and surrounding area with a front to back downward motion.
            d. Discard towelette into the trash.
            e. Repeat cleansing process 2 more times using a clean towelette each time. Remember to hold the
                foreskin back throughout cleansing process.
            f. Begin to urinate into the toilet. Stop and place the collection container into position and then begin
               to urinate again into the container. The container should not touch the penis. It is extremely
               important not to touch the inside or the lip of the container with the hands or any other part of
               the body.
            g. After the specimen has been collected secure the lid on tightly and give the specimen to the
                lab personnel.
                     i. The patient should be instructed not to touch the interior of the top orcontainer.
                    ii. Label the cup with the patient information.
                   iii. Urinate into the urine specimen container.
                   iv. Collect the sample until the cup is about ¾‘s full.
                    v. Transfer sample to gray top urine culture tube labeled with patient information.
E. Nasal Culture:
      1. Insert a premoistened swab about 2 cm into the nose. Rotate the swab against the nasal mucosa.
      2. Submit the swab in sterile transport media to the laboratory immediately.
      3. Stable at room temperature for 24 hours.
      4. Refrigerated, not established.
      5. Frozen specimens are not acceptable for testing.
F. Throat Culture:
      1. Obtain the throat swab after depressing the tongue with a tongue depressor.
      2. Sample the posterior pharynx, tonsils, and inflamed areas.
      3. Submit the swab in sterile transport medium to the laboratory immediately.
      4. Stable at room temperature for 24 hours.
      5. Refrigerated and frozen specimens are not acceptable for testing.
G. Group B Strep Culture:
      1. Obtain the vaginal swab using a culture transport swab.
      2. Wipe away any excessive amount of secretion or discharge from the vaginalarea.
      3. Carefully insert the swab into the lower one-third part of the vagina, and sample secretions from
          the mucosa.
      4. Carefully insert the same swab, approximately 2.5 cm beyond the anal sphincter, and gently rotate
          to sample anal crypts.
      5. Replace the swab in its container and submit to the laboratory immediately.
      6. Stable at room temperature for 24 hours.
      7. Stable refrigerated for 6 days.
      8. Frozen specimens are not acceptable for testing.

IV. QUALITY CONTROL

N/A

V. CALCULATIONS/CALIBRATION

N/A

VI. INTERPRETATIONS

N/A

VII. METHOD PERFORMANCE SPECIFICATIONS

N/A

VIII. REFERENCES

Isenberg HD, Clinical Microbiology Procedures Handbook. 2nd ed. Vol 1. Washington, DC: American Society for Microbiology Press; 2004.

IX. RELATED DOCUMENTS

N/A

X. DOCUMENT HISTORY

MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

X - MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

New Document Control Format. New procedure.



Laboratory Director

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Natalie Depcik-Smith, MD
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Department Director

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