50.0 Collection and Handling of Urine Specimens


Title of Manual: Specimen Collection

Version No: 1.0

Title: Collection and Handling of Urine Specimens

Effective Date: 12/20/10
Revised Date: 06/23/14

Document #: GPA.SPC.50.0


I. PURPOSE

To provide directions for the proper collection of urine specimens for urine analysis and urine cultures.

This is to be performed by all personnel responsible for collecting urine specimens for urine analysis.

II. MATERIALS

Reagents

Supplies

Equipment

• Sterile urine specimen container.
• Cleansing towelettes


III. PROCEDURE

Routine Urine Specimens:
1. Provide the patient with a clean urine collection cup with their two unique identifiers written on the

    container (i.e. patients last, first name and DOB)
2. Instruct the patient to urinate in the container. 6-12 mL of urine is needed for an adequate
    microscopic analysis.
3. Specimens should be refrigerated if not tested within 2 hours of collection. Refrigeration will extend
    testing for up to 24 hours. The specimens may be sub-optimal if it is >24 hours old and or not
    refrigerated after 2 hours of collection.

Collection of Clean Catch Midstream:
Instructions for Female Patient:

a. Remove all necessary garments.
b. Wash hands thoroughly with soap and warm water, rinse and dry hands.
c. Separate the skin folds around the urinary opening and clean this area with the provided cleaning towelette.
    Clean the opening and the surrounding area in a front to back downward motion.
d. Discard the towelette in the trash.
e. Repeat the cleansing process 2 more times using clean towelettes each time. Remember to hold the skin
    folds apart while cleansing the area.
f. Holding the skin folds apart with one hand, begin to urinate into the toilet, and then stop urinating and place
   the specimen cup into position and begin urinating again. It is extremely important not to touch the inside
   or the lip of the container with your hands or any part of the body.
g. After specimen is collected, secure the lid on tightly and give the specimen to the lab personnel.

Instructions for the Male Patient:
a. Remove all necessary garments.
b. Wash hands thoroughly with soap and water, rinse and dry hands.
c. Pulling the foreskin back with one hand, clean the head of the penis with the provided cleansing towelette.
   Clean the opening and surrounding area with a front to back downward motion.
d. Discard towelette into the trash.
e. Repeat cleansing process 2 more times using a clean towelette each time. Remember to hold the foreskin
    back throughout cleansing process.
f. Begin to urinate into the toilet. Stop and place the collection container into position and then begin to    urinate again into the container. The container should not touch the penis. It is extremely important not
   to touch the inside or the lip of the container with the hands or any other part of the body
g. After the specimen has been collected secure the lid on tightly and give the specimen to the lab personnel.

Collection of 24 Hour Specimen (Patient Instructions):
a. The patient will be given a container by the physician or lab personnel.
b. The morning of the patient is to start collection the 24 hour specimen; bladder should be emptied as usual.
    DO NOT put this specimen into the container.
c. All specimens, day and night, after the first morning void should be collected into another clean container
   and transferred the 24 hour urine collection container.
d. The collection will end after the first morning void is collected in the 24 hour collection container.
e. Keep the 24 hour collection container in the refrigerator or on ice during the entire collection process.
f. If a urine collection is missed please notify your physician and the laboratory personnel.
g. The urine and the request form should be delivered to the laboratory as soon after collection as possible.

Collection of Urine Culture:
a. See collection of clean-catch, mid stream urine procedure.
b. The patient should be instructed not to touch the interior of the top or container.
c. Label the cup with the patient information.
d. Urinate into the urine specimen container.
e. Collect the sample until the cup is about ¾‘s full.
f. Transfer sample to gray top urine culture tube labeled with patient information.

NOTE:
If the physician has written an order for 24 hour urine for Creatinine Clearance you will need to also collect 1 SST tube. The requisition will also require a total volume of urine that was collected in that 24 hour period. The volume can be determined by the marks on the 24 hour urine container.

IV. QUALITY CONTROL

N/A

V. CALCULATIONS/CALIBRATION

N/A

VI. INTERPRETATIONS

N/A

VII. METHOD PERFORMANCE SPECIFICATIONS

N/A

VIII. REFERENCES

Urinalysis and Collection, Transportation, and Preservation of Urine Specimens;
2nd Edition: GP16 – A2, Vol. 21, Wayne, PA. 2001

IX. RELATED DOCUMENTS

N/A

X. DOCUMENT HISTORY

X - MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

2/14/14 New Document Control Format

6/23/14 Moved procedure to
Specimen Collection Manual



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date

Robert M. Gay, M.D.
(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)



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