44.0 Pediatric Venipuncture


Title of Manual: Specimen Collection

Version No: 1.0

Title: Pediatric Venipuncture Procedure

Effective Date: 12/20/10
Revised Date: 06/23/14

Document #: GPA.SPC.44.0


I. PURPOSE

To provide guidelines for obtaining samples from pediatric patients, especially infants also see Capillary Blood Collection Procedure.

This is to be performed by all personnel who are authorized and trained to perform phlebotomy.

II. MATERIALS

Reagents

Supplies

Equipment

● Butterfly needle
● Blood Collection Tubes
● Alcohol Prep Pads
● 2x2 Non-Sterile Gauze
Pads
● Tourniquet
● Sharps Container
● Bandages or Tape


III. PROCEDURE

1. Identify the infant patient.
2. Assemble appropriate equipment.
3. Infants under one year of age or under 22 pounds should have blood collected by heel stick
    capillary puncture.
4. Unless absolutely necessary or the physician specifically orders a peripheral draw, venipuncture should
    not be performed on children under one year of age.

Children / Infants 6-100 pounds:
1. Pediatric microtainer tubes
2. Maximum tube fill – 3ml
3. Maximum blood draw at one time:

Weight

Max Blood Draw

Weight

Max Blood Draw

6-8 lbs

2.5 mL

41-60 lbs

20 mL

8-10 lbs

3.5 mL

61-65 lbs

25 mL

11-15 lbs

5.0 mL

66-100 lbs

30 mL

16-40 lbs

10 mL

 

 


4. It is critical that blood volumes withdrawn on newborns and infants be minimal to prevent
    iatrogenic anemia.
5. Children over 100 pounds, adult blood volumes may be collected.

Restraining Small Children:
1. When working with combative children who cannot remain still, considerations must be made to prevent
    harm to the child as well as preventing accidental needle sticks to the phlebotomist and assistants.
        a. Work out a plan with the parent and assistant prior to the child being brought into the phlebotomy area.
        b. Plan for immobilizing the arm to be stuck, as well as, free arm and legs.
        c. Prepare equipment out of sight and out of reach from the child.
        d. Distract the child from the procedure. Give them something to visually focus on away from the arm
            the collection will take place is possible.
2. Often the child can sit in the parents lap and the parent holds the child’s free arm with one hand and can
    keep the sticking arm steady with the other hand. When the parent cannot restrain the child satisfactorily
    and the parent gives consent, a papoose board may be used if one is available.

IV. QUALITY CONTROL

N/A

V. CALCULATIONS/CALIBRATION

N/A

VI. INTERPRETATIONS

N/A

VII. METHOD PERFORMANCE SPECIFICATIONS

N/A

VIII. REFERENCES

Procedures for the collection of Diagnostic Blood Specimens by Venipuncture;
Approved Standard – 5th Edition NCCLS, Wayne, PA, 2003 Vol. 23, H3-A5.

IX. RELATED DOCUMENTS

N/A

X. DOCUMENT HISTORY

X - MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

02/10/14 New Document Control Format

06/23/14 Moved procedure to
Specimen Collection Manual



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date

Robert M. Gay, M.D.
(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)



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