30.0 Sputum


Title of Manual: Specimen Collection

Version No: 1.0

Title: Sputum

Effective Date: 11/01/09
Revised Date: 01/02/14

Document #: GPA.SPC.30.0


I. PURPOSE

To provide the proper collection protocol for Sputum testing.

II. MATERIALS

Reagents

Supplies

Equipment

Bronchial secretion:
• Sterile specimen cup or
Cytolyt preservative fluid.


III. PROCEDURE

1. Label container with patient name, date of birth and source / site.
2. Have patient rinse out their mouth with water before collection.
3. Sputum should be expectorated directly into the specimen container.
4. The patient should be instructed that mucous or saliva is not satisfactory and that a deep cough is
    absolutely essential to be of diagnostic value.
5. Indicate to the patient that the fluid in the specimen container is not for internal consumption.
6. Sputum may be collected fresh or in Cytolyt.
7. First morning specimens are preferred. It is not necessary to obtain a four-hour sputum.
8. After the sample is collected replace the lid tightly.
9. The cytology specimen cannot be used for culture.
10. Place container in a specimen biohazard bag with the requisition in the side pocket.

IV. QUALITY CONTROL

• Minimum Volume:
    • N/A
• Storage Requirements:
    • Refrigerate and transport to the cytology department as soon as possible.
• Stability Requirements:
    • 24 hours

V. CALCULATIONS/CALIBRATION

VI. INTERPRETATIONS

VII. METHOD PERFORMANCE SPECIFICATIONS

VIII. REFERENCES

IX. RELATED DOCUMENTS

X. DOCUMENT HISTORY

MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

X - MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

1/2/14 New Document Control Format



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date


(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)



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