20.0 Conventional Pap Smear


Title of Manual: Specimen Collection

Version No: 1.0

Title: Conventional Pap Smear

Effective Date: 11/01/09
Revised Date: 01/02/14

Document #: GPA.SPC.19.0


I. PURPOSE

To provide the proper collection protocol for Pap Smear testing.

II. MATERIALS

Reagents

Supplies

Equipment

Cervical sampling:
• CytoBrush & Spatula
on 1 slide smear


III. PROCEDURE

1. Label slide with patient’s first and last name in pencil.
2. Do not utilize a lubricant on the speculum.
3. Remove the mucus plug with a cotton swab. Discard the cotton swab.
4. Ectocervix Sample – Insert spatula into the cervical os and rotate one full turn (360 degrees). Smear
    thinly on ½ of the slide and fix immediately before air-drying occurs. You may cover unused portion of
    slide with paper toweling to prevent fixative from getting onto that surface.
5. Endocervix Sample – Insert cytobrush, (recommended to obtain Endocervical cells) into Endocervix.     Gently rotate ½ turn (180 degrees). Using moderate pressure, roll on remaining ½ of the slide (make
    certain to complete 360 degree turn) and fix immediately. Spray fixative onto the slide two to four sprays,
    10 inches from the slide. Failure to fix immediately could result in the Pap smear being unsatisfactory
    for evaluation.
6. Vaginal Pool – A lateral vaginal pool sample is recommended for a Maturation Index or if endometrial
    cancer is suspected and/or for women 40 years and older. Collect vaginal pool mucus using a spatula; place
    it on a second slide. Fix immediately with spray fixative.
7. Place the slides inside slide protector and into a biohazard specimen bag for transport. Place the
    completed requisition in the side pocket of the specimen bag.

IV. QUALITY CONTROL

• Minimum Volume:
    • N/A
• Storage Requirements:
    • Room Temperature
• Stability Requirements:
    • 3 Weeks

V. CALCULATIONS/CALIBRATION

VI. INTERPRETATIONS

VII. METHOD PERFORMANCE SPECIFICATIONS

VIII. REFERENCES

IX. RELATED DOCUMENTS

X. DOCUMENT HISTORY

MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

X - MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

1/02/14 New Document Control Format



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date


(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)



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