11.0 Breast Secretions (Nipple Discharge)


Title of Manual: Specimen Collection

Version No: 1.1

Title: Breast Secretions (Nipple Discharge)

Effective Date: 11/01/09
Revised Date: 01/02/14

Document #: GPA.SPC.11.0


I. PURPOSE

To provide the proper collection protocol for breast secretion testing.

II. MATERIALS

Reagents

Supplies

Equipment

Fluid discharge:
• 2 slide smears
• Sterile Cup
Preservcyt
Cytolyt Vial


III. PROCEDURE

In-Office Smear:
1. Label slides with patient’s name, date of birth and source site on the frosted end in pencil.
2. Express a small amount of fluid from the nipple.
3. Touch the slide to the nipple.
4. Place the other slide on top of the first slide with specimen. Spread the glass slides apart from each other
    to evenly distribute cells. Immediately fix one smear with spray fixative marked (F) on the slide label and
    the other let air dry marked (AD). Two or more slides may be made.
5. Place slides in appropriate cardboard/paper holder and secure with tape.
6. Place in a biohazard bag along with the completed requisition in the side pocket of the specimen bag.
7.


Sterile Cup/Cytolyt/Preservcyt:
Another method for collection is through the use of a sterile cup, cytolyt or preservcyt vial. For this collection, express an ample amount of fluid from nipple into one of the before mention collection containers. A sterile cup may be used initially with the addition of either preservcyt or cytolyt poured into the cup for preservation. If the later mentioned method is used, the container must be properly labeled with the preservative used for proper laboratory processing.

The use of the collection containers can be used in conjunction with smears.

IV. QUALITY CONTROL

Storage Requirements:
    Slides & Preservative Fluid - Room Temperature
    Fresh in sterile cup – Refrigerated
Stability Requirements:
    3 weeks is slide or preservative
    24 hours if fresh specimen

V. CALCULATIONS/CALIBRATION

VI. INTERPRETATIONS

VII. METHOD PERFORMANCE SPECIFICATIONS

VIII. REFERENCES

IX. RELATED DOCUMENTS

X. DOCUMENT HISTORY

MINOR REVISION
(Laboratory Director’s Signature on Original Subsequent Document Attached)

X - MAJOR REVISION
(Requires Laboratory Director & Department Director Signature - where applicable)

Reason for Change

1/02/14 New Document Control Format



Laboratory Director

(Signature)

Date

Natalie Depcik-Smith, MD
(Print)



Department Director

(Signature)

Date


(Print)



Designee

Date

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)

(Print)

(Signature)



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